STATE OF GLUTATHIONE ANTIOXIDANT SYSTEM IN BLOOD LYMPHOCYTES AT OVARIAN CANCER (12-15)

STATE OF GLUTATHIONE ANTIOXIDANT SYSTEM IN BLOOD LYMPHOCYTES AT OVARIAN CANCER (12-15)

Выбор валюты
Дата публикации статьи в журнале: 2020/11/09
Название журнала: Американский Научный Журнал, Выпуск: 42, Том: 1, Страницы в выпуске: 12-15
Автор:
Lviv, Ukraine , Danylo Halytsky Lviv National Medical University, Graduate Student
Автор: Onufrovych O.
Lviv, Ukraine , Danylo Halytsky Lviv National Medical University, Assistant Professor, PhD
Автор: Fafula R.
Lviv, Ukraine , Danylo Halytsky Lviv National Medical University, Professor, D Sci
Анотация: Activation of lipid peroxidation (LP) processes in blood lymphocytes of women with ovarian cancer was shown. Thus, in the control group the concentration of malonic dialdehyde (MDA) in blood lymphocytes was (62.1±4.5) μmol/mg protein. This value increases significantly up to (96.9±7.4) μmol/mg protein at ovarian cancer (OC). A similar situation is observed when determining the concentration of MDA in serum. Thus, in the control group it reaches (7.5±0.6) μmol/L. When OC, the processes of LP are intensified in 1.6 times relative to the control group. Simultaneously, with the intensification of LP processes the corresponding changes in the activity of enzymes of the glutathione antioxidant system were revealed. It was shown that in practically healthy women the concentration of glutathione is (17.8±1.5) nmol/mg protein. At OC, this value increases in 1.4 times and reaches (25.3±2.2) nmol/mg protein. Also a significant difference in glutathione peroxidase activities was revealed. In the control group it is (161.8±12.7) nmol GSH/min∙mg protein. At OC this value decreases in 1.7 times up to (96.1±8.8) nmol GSH/min∙mg of protein. Regarding the activity of glutathione reductase, in the lymphocytes of the control group it is equal to (51.9±5.1) nmol NADPH/min∙mg protein. At OC, this activity is reduced to (35.1±3.1) nmol NADPH/min∙mg protein, that is in 1.4 times lower than in control group. The glutathione transferases (GSTs) activity of the control group is (114.9±9.2) nmol GSH/min∙mg protein. With the development of OC (stage III-IV) the activity of GSTs increases to (138.8±11.6) nmol GSH/min∙mg of protein, that is in 1.2 times more than in control, however these changes are not statistically significant. Thus, OC significantly intensifies LP processes in both lymphocytes and blood plasma. Simultaneously, the development of OC significantly reduces the activity of two major antioxidant enzymes - glutathione peroxidase and glutathione reductase.
DOI:
Данные для цитирования: Barylyak R. Onufrovych O. Fafula R.. STATE OF GLUTATHIONE ANTIOXIDANT SYSTEM IN BLOOD LYMPHOCYTES AT OVARIAN CANCER (12-15). Американский Научный Журнал. Медицинские науки. 2020/11/09; 42(1):12-15.

Список литературы: 1. Bandebuche S, Melinkeri RR. Oxidative stress and antioxidant status in patients of ovarian cancer. Biomedical Research. 2011;22(2):115-122. 2. Caglayan A, Katlan DC, Tuncer ZS et al. Impaired antioxidant enzyme function with increased lipid peroxidation in epithelial ovarian cancer. IUBMB Life. 2017;69(10):802-813. 3. Caglayan A, Katlan DC, Tuncer ZS, Yüce K.J. Evaluation of trace elements associated with antioxidant enzymes in blood of primary epithelial ovarian cancer patients. Trace Elem Med Biol. 2019;52:254-262. 4. Calaf GM, Urzua U, Termini L, Aguayo F. Oxidative stress in female cancers. Oncotarget. 2018;9(34):23834-23842. 5. Handayani E, Edianto D, Sahil MF, Tobing L. Glutathione peroxidase in ovarian cancer patients in Indonesia. South African Journal of Obstetrics and Gynaecology. 2019;25(51):41-52. 6. Fafula RV, Iefremova UP, Luchkovska NE, Vorobets ZD, Kulachkovskii OR. Methodological approach to the study of the enzymatic spectrum of lymphocytes at pathological states using a detergent of saponin (ultrastructural study). Visnyk Problem Biologii i Medecini. 2012;1(96):163-166. 7. Kruk J, Aboul-Enein HY. Reactive oxygen and nitrogen species in carcinogenesis: implications of oxidative stress on the progression and development of several cancer types. Mini Rev. Med. Chem. 2017;17(11):904-919. 8. Markman M, Webster K, Zanotti K. Examples of the marked variability in the relationship between the serum CA-125 antigen level and cancer-related symptoms in ovarian cancer. Gyn. Onc. 2004;93(3):715–117. 9. Wang S, He G, Chen M. The role of antioxidant enzymes in the ovaries. Oxidative Medicine and Cellular Longevity. 2017;2017:1-14. 10. Worley BL, Kim YS, Mardini J et al. GPx3 supports ovarian cancer progression by manipulating the extracellular redox environment. Redox Biology. 2019;25:1-9. 11.Zhang M-Li, Wu H-T, Chen W-J et al. Involvement of glutathione peroxidase in the occurrence and development of breast cancers. J. Transl. Med. 2020;18:247-252. 12.Zhao G, Cardenas H, Matei D. Ovarian cancer – why lipids matter. Cancers (Basel). 2019;11(12):1877-1882.